Cov khoom

Ib qho rhiab heev microRNA (miRNA) biosensor uas ntiav ruthenium oxide nanoparticle (RuO(2) NP)-pib polymerization ntawm 3,3'-dimethoxybenzidine (DB) thiab miRNA-templated deposition ntawm insulating poly (3,3'-dimethoxybenzidine) (PDB) zaj duab xis tau piav qhia hauv txoj haujlwm no.Lub biosensor tau ua los ntawm ib qho sib xyaw monolayer ntawm oligonucleotide capture probes (CPs) thiab 4-mercaptoaniline ntawm lub electrode kub.Tom qab hybridization nrog RuO(2) NP-tagged lub hom phiaj miRNA, sib tov ntawm DB/H(2)O(2) hauv pH 5.0 0.10 M acetate buffer tau siv rau biosensor.RuO(2) NPs ua haujlwm ua polymerization pib / catalyst rau polymerization ntawm DB.Thiab cov hybridized anionic miRNA strands thiab CPs dawb ua cov qauv, coj kev tso nyiaj ntawm PDB.Tus nqi ntawm PDB tso nyiaj thiab nws cov hluav taws xob insulating ncaj qha mus rau qhov concentration ntawm lub hom phiaj miRNA hauv kev daws.Electrochemical impedance spectroscopic kev ntsuam xyuas pom tau hais tias ib tug linear charge-transfer resistance-concentration kev sib raug zoo los ntawm 6 .0 fM mus rau 2.0 pM tau txais tom qab 60 min ntawm incubation nyob rau hauv DB / H (2) O (2) sib tov.Tsis muaj kev sib xyaw ua ke ntawm pre-miRNA thiab miRNA paub tab thiab me me hla-hybridization ntawm cov neeg nyob sib ze miRNA tsev neeg txawm nyob rau ntawm ib theem-piv-mismatched.Qhov no impedance-based biosensor muaj lwm txoj hauv kev zoo nkauj rau miRNA kev qhia profileing thiab tuaj yeem ua rau kev txhim kho ntawm cov khoom siv sib txawv ntawm miRNA profileing system.