Cov khoom

Kev sib txawv ntawm orbital fibroblasts rau hauv cov laus adipocytes thiab tom qab sib sau ntawm cov ntaub so ntswg adipose tau pom nyob rau hauv kev loj hlob ntawm Graves 'orbitopathy (GO).Autophagy koom nrog hauv adipogenesis, tab sis me ntsis paub txog lub luag haujlwm ntawm autophagy hauv kev pib thiab kev loj hlob ntawm GO.Lub hom phiaj ntawm txoj kev tshawb no yog los tshawb xyuas lub luag haujlwm ntawm autophagy nyob rau hauv pathogenesis ntawm GO.Orbital adipose / connective cov ntaub so ntswg explants los ntawm cov neeg mob GO thiab los ntawm ib txwm kawm, raws li zoo raws li cais orbital fibroblasts, tau soj ntsuam.Adipogenesis raug ntxias siv qhov sib txawv nruab nrab nrog lossis tsis muaj hydrogen peroxide, thiab autophagy tau tswj hwm siv bafilomycin A1 thiab Atg5-targeted luv hairpin RNA (shRNA).Autophagosomes tau txheeb xyuas los ntawm electron microscopy.Kev nthuav qhia ntawm cov noob caj noob ces thiab adipogenesis ntsig txog kev hloov pauv tau raug tshuaj xyuas los ntawm lub sijhawm hloov pauv-polymerase saw cov tshuaj tiv thaiv thiab / lossis Western blot tsom xam.Lipid droplet tsub zuj zuj yog kuaj los ntawm Roj Liab O staining.Autophagic vacuoles muaj ntau nyob rau hauv GO hlwb dua li nyob rau hauv non-GO hlwb (p<0.05).Kev nthuav qhia ntawm autophagy-hais txog cov noob tau nce siab dua hauv GO cov ntaub so ntswg thiab cov hlwb ntau dua li ntawm lawv cov neeg tsis yog GO, feem.Interleukin-1β nce LC3-II, p62, thiab Atg7 protein ntau hauv GO hlwb.Autophagosome tsub zuj zuj tau pom nyob rau hnub 4 ntawm adipogenesis thiab txo qis los ntawm hnub 10, nrog rau kev tsim cov lipid droplet.Kev nthuav qhia ntawm LC3 thiab p62 proteins nce nyob rau hauv 48 teev ntawm kev sib txawv thiab ploj zuj zus los ntawm hnub 4 txog 10. Kev kho mob Bafilomycin A1 thiab Atg5 knockdown los ntawm shRNA inhibited lipid droplet tsub zuj zuj thiab suppressed qhia ntawm adipogenic cov cim. cov ntaub so ntswg tsis-GO thiab cov hlwb, qhia tias autophagy plays lub luag haujlwm hauv GO pathogenesis.Autophagy manipulation tej zaum yuav yog lub hom phiaj kho rau GO.