p-Nitrophenyl -aL-Fucopyranoside Cas: 10231-84-2 Dawb rau daj daj crystalline hmoov
| Catalog Number | xwm 90142 |
| Khoom npe | p-Nitrophenyl-aL-Fucopyranoside |
| CAS | 10231-84-2 |
| Molecular Formula | C12H15NO7 |
| Molecular Luj | 285.25 Nws |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
| Harmonized tariff Code | 29 400 000 IB |
Khoom Specification
| Qhov tshwm sim | Dawb mus daj daj crystalline hmoov |
| Assay | 99% |
| TLC | Ib qho chaw |
| Purity HPLC | Tsawg tshaj 98% |
| Solubility (1% hauv dej) | Clear colorless tov |
| Qhov ntom | 1.503 ± 0.06 g / cm3 (20 ºC 760 Torr), |
| Melting point | 196-197 ºC |
| Boiling point | 515.4 ° Cat760mmHg |
| Flash point | 265.5°C dej |
| Refractive Index | 1.623 ib |
LecA (PA-IL) yog cytotoxic lectin thiab adhesin uas tsim los ntawm Pseudomonas aeruginosa uas khi hydrophobic galactosides nrog siab tshwj xeeb thiab affinity.Los ntawm kev siv lecA-egfp kev txhais lus fusion thiab immunoblot tsom xam ntawm biofilm extracellular matrix, peb pom tias lecA tau qhia hauv biofilm-loj hlwb.Hauv kev soj ntsuam biofilm zoo li qub ntawm ob qho tib si polystyrene thiab stainless hlau, biofilm qhov tob thiab qhov chaw npog tau raug txo los ntawm kev hloov pauv ntawm lecA thiab txhim kho hauv LecA-overproducing strain PAO-P47.Biofilm nto npog los ntawm niam txiv hom, PAO-P47 tab sis tsis yog lecA mutant ntawm steel daim coupons kuj inhibited los ntawm kev loj hlob nyob rau hauv lub xub ntiag ntawm ob isopropyl-beta-D-thiogalactoside (IPTG) los yog p-nitrophenyl-alpha-D-galactoside ( NPE).Tsis tas li ntawd, cov neeg laus cov tsiaj qus-hom biofilms tsim thaum tsis muaj cov hydrophobic galactosides tuaj yeem tawg los ntawm kev sib ntxiv ntawm IPTG.Hauv qhov sib piv, ntxiv ntawm p-nitrophenyl-alpha-L-fucose (NPF) uas muaj kev sib raug zoo rau P. aeruginosa LecB (PA-IIL) lecti n tsis muaj kev cuam tshuam rau biofilm tsim los yog dispersal.Planktonic kev loj hlob ntawm P. aeruginosa PAO1 tsis muaj kev cuam tshuam los ntawm qhov muaj IPTG, NPG lossis NPF, thiab tsis muaj kev siv cov suab thaj no los ua cov pa roj carbon monoxide, tawm tswv yim tias cov teebmeem tshwm sim ntawm kev tsim biofilm yog vim muaj kev sib tw inhibition ntawm LecA-ligand. kev khi.Cov txiaj ntsig zoo sib xws kuj tau txais rau biofilms loj hlob nyob rau hauv dynamic flow condition ntawm steel daim coupons, qhia tias LecA pab txhawb rau P. aeruginosa biofilm architecture nyob rau hauv txawv ib puag ncig tej yam kev mob.
Ib qho alpha-L-fucosidase (EC 3.2.1.51) muaj peev xwm tso tawm t-fucosyl residue los ntawm sab saw ntawm xyloglucan oligosaccharides tau kuaj pom nyob rau hauv nplooj ntawm Arabidopsis nroj tsuag.Ntxiv mus, ib qho alpha-L-fucosidase nrog cov substrate tshwj xeeb zoo sib xws tau ntxuav los ntawm cov zaub qhwv (Brassica oleracea) nplooj los ua ib pawg ntawm SDS-PAGE.Ob qhov peptide ib ntus tau txais los ntawm cov kab protein no, thiab lawv tau siv los txheeb xyuas Arabidopsis gene coding rau alpha-fucosidase uas peb thov hu rau AtFXG1.Tsis tas li ntawd, ib qho Arabidopsis noob nrog homology nrog paub alpha-L-fucosidases kuj tau pom, thiab peb tau thov kom hu nws ua AtFUC1.Ob qho tib si AtFXG1 thiab ATFUC1 tau hais tawm heterologously hauv Pichia pastoris hlwb thiab cov dej num alpha-L-fucosidase secreted rau cov kab lis kev cai nruab nrab.Lub alpha-L-fucosidase encoded los ntawm AtFXG1 yog nquag tawm tsam oligosaccharides los ntawm xyloglucan XXFG nrog rau 2'-fucosyl-lactitol tab sis tsis tawm tsam p-nitrophenyl-alpha-L-fucopyranoside.Txawm li cas los xij, AtFUC1 heterologously tau hais tawm tsuas yog tawm tsam 2'-fucosyl-lactitol.Yog li, tus qub yuav tsum muaj feem xyuam rau xyloglucan metabolism.
