L-Proline Cas: 147-85-3 99% Dawb hmoov
| Catalog Number | xwm 90293 |
| Khoom npe | L-Proline |
| CAS | 147-85-3 : kuv |
| Molecular Formula | C5H9 NPO 2 |
| Molecular Luj | 115.13046 |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 2933980 :ib |
Khoom Specification
| Assay | 99% min |
| Qhov tshwm sim | Dawb hmoov |
| Kev sib hloov tshwj xeeb | -84.5 -86 |
| Cov hlau hnyav | <15ppm |
| AS | <1 ppm |
| Ph | 5.9-6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Poob rau ziab | <0.3% |
| Residue ntawm Ignition | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Lub Xeev Txoj Cai | > 98% |
Nkag siab txog cov metabolism hauv microbial party yog qhov tseem ceeb rau kev txhim kho thiab kev ua kom zoo ntawm tag nrho cov cell raws li biocatalytic txheej txheem, raws li nws dictates ntau lawm efficiency.Qhov no yog tshwj xeeb tshaj yog muaj tseeb rau redox biocatalysis qhov twg metabolically active hlwb ua hauj lwm vim lub cofactor / cosubstrate regenerative muaj peev xwm endogenous nyob rau hauv lub party.Recombinant Escherichia coli tau siv los ua ntau tshaj proline-4-hydroxylase (P4H), dioxygenase catalyzing hydroxylation ntawm dawb L-proline rau hauv trans-4-hydroxy-L-proline nrog a-ketoglutarate (a-KG) ua cosubstrate.Nyob rau hauv tag nrho-cell biocatalyst no, central carbon metabolism muab qhov yuav tsum tau cosubstrate a-KG, coupling P4H biocatalytic kev ua tau zoo ncaj qha rau carbon metabolism thiab metabolic kev ua si.Los ntawm kev siv ob qho kev sim thiab suav cov cuab yeej biology, xws li metabolic engineering thiab (13) C-metabolic flux analysis ((13) C-MFA), peb tau tshawb xyuas thiab piav qhia ntau npaum li cas ntawm lub cev, metabolic, thiab bioenergetic teb ntawm tag nrho cov cell biocatalyst. mus rau lub hom phiaj bioconversion thiab txheeb xyuas tau metabolic bottlenecks rau ntxiv rational pathway engineering.A proline degradation-deficient E. coli hom yog tsim los ntawm deleting lub putA noob encoding proline dehydrogenase.Tag nrho-cell biotransformations nrog rau qhov kev hloov pauv no coj tsis tau tsuas yog rau qhov ntau ntawm proline hydroxylation tab sis kuj ua rau ob npaug ntawm qhov tshwj xeeb trans-4-L-hydroxyproline (hyp) tsim tus nqi, piv rau hom tsiaj qus.Kev soj ntsuam ntawm cov pa roj carbon flux los ntawm cov metabolism hauv nruab nrab ntawm cov kev hloov pauv tau qhia tias qhov nce a-KG thov rau P4H kev ua haujlwm tsis tau txhim kho a-KG generating flux, qhia txog kev tswj hwm TCA kev ua haujlwm nruj raws li cov xwm txheej tau kawm.Hauv hom tsiaj qus, P4H synthesis thiab catalysis ua rau txo qis hauv biomass tawm los.Interestingly, ΔputA strain ntxiv tau them nyiaj rau qhov cuam tshuam ntawm ATP thiab NADH poob los ntawm kev txo cov kev xav tau ntawm lub zog ntawm kev sib piv cov piam thaj qis qis, es tsis txhob ua rau TCA kev ua si.Qhov putA knockout hauv recombinant E. coli BL21(DE3)(pLysS) tau pom. tau cog lus rau cov khoom tsim tau P4H catalysis tsis yog nyob rau hauv cov nqe lus ntawm biotransformation tawm los, tab sis kuj hais txog tus nqi rau biotransformation thiab proline uptake thiab yield ntawm hyp ntawm lub zog qhov chaw.Cov txiaj ntsig tau qhia tias, tom qab kev sib tw, kev sib txuas ntawm TCA-cycle mus rau proline hydroxylation ntawm cosubstrate a-KG dhau los ua qhov tseem ceeb ntawm kev txwv thiab lub hom phiaj txhawm rau txhim kho kev ua tau zoo ntawm a-KG-dependent biotransformations.
