L-Leucine Cas: 61-90-5 99% Dawb hmoov
| Catalog Number | xwm 90304 |
| Khoom npe | L-Leucine |
| CAS | 61-90-5 : kuv |
| Molecular Formula | C6H13NO2 |
| Molecular Luj | 131.17292 ib |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 29224985 ib |
Khoom Specification
Zoo ib yam li lwm cov kab mob zoo-strand RNA, tombusviruses tau rov ua dua los ntawm cov kab mob sib kis kab mob sib kis (VRC).Lub VRC muaj p92 tus kab mob-coded RNA-dependent RNA polymerase (RdRp), tus kab mob p33 RNA chaperone, thiab ob peb co-opted host proteins.Txhawm rau dhau los ua qhov ua haujlwm RdRp tom qab nws txhais lus, p92 replication protein yuav tsum tau muab tso rau hauv VRC, ua raws li nws qhov ua kom.Peb tau pom yav dhau los hauv qhov kev ntsuam xyuas ntawm tes-dawb cov poov xab extract-raws li kev ua kom tus kab mob Tomato bushy stunt virus (TBSV) RdRp yuav tsum muaj cov tswv tsev soluble (s).Hauv tsab xov xwm no, peb txheeb xyuas cov cellular heat shock protein 70 (Hsp70) raws li kev sib koom ua ke tus tswv tsev tsim nyog rau kev ua kom muaj N-terminally truncated recombinant TBSV RdRp.Tsis tas li ntawd, me me-molecule-based thaiv ntawm Hsp70 muaj nuj nqi inhibits RNA synthesis los ntawm tombusvirus RdRp hauv vitro.Tsis tas li ntawd, peb qhia tau hais tias nruab nrab phospholipids, uas yog, phosphatidylethanolamine (PE) thiab phosphatidylcholine (PC), txhim khu RdRp activation hauv vitro.Hauv qhov sib piv, phosphatidylglycerol (PG) qhia tau hais tias muaj zog thiab tseem ceeb inhibitory cuam tshuam rau hauv vitro RdRp ua kom.Peb kuj qhia tau tias PE thiab PC txhawb nqa RdRp-viral ntxiv-strand RNA [(+)RNA] kev sib cuam tshuam, thaum PG inhibits kev khi ntawm tus kab mob RNA rau RdRp.Raws li cov stimulatory tiv thaiv inhibitory luag hauj lwm ntawm ntau yam phospholipids nyob rau hauv tombusvirus RdRp ua kom, peb npaj siab hais tias cov lipid muaj pes tsawg leeg ntawm lub hom phiaj subcellular membranes tej zaum yuav siv los ntawm tombusviruses los tswj VRC tshiab sib dhos thaum lub sij hawm kis kab mob.Cov kab mob-coded RNA-dependent RNA polymerase. (RdRp), uas yog lub luag haujlwm rau kev sib txuas cov kab mob RNA progeny hauv cov kab mob kis ntawm ntau tus kab mob RNA zoo, pib tsis ua haujlwm.Lub tswv yim no yuav ua rau kom tsis txhob kis tus kab mob RNA synthesis hauv cytosol uas yuav ua rau sai sai rau kev cuam tshuam ntawm RNA-triggered cellular antiviral teb.Thaum lub sij hawm sib dhos ntawm cov membrane-bound replicase complex, tus kab mob RdRp ua haujlwm los ntawm kev nkag siab tsis tiav uas ua rau RdRp muaj peev xwm ntawm RNA synthesis.Los ntawm kev siv TBSV RdRp, peb qhia tau hais tias cov co-opted cellular Hsp70 chaperone thiab nruab nrab phospholipids pab txhawb RdRp activation nyob rau hauv vitro.Nyob rau hauv sib piv, phosphatidylglycerol (PG) muaj ib tug tseem ceeb inhibitory nyhuv nyob rau hauv vitro RdRp activation thiab RdRp-viral RNA kev sib cuam tshuam, qhia hais tias lub membranous microdomain nyob ib ncig ntawm lub RdRp zoo heev cuam tshuam nws lub peev xwm rau RNA synthesis.Yog li, kev ua kom tus kab mob RdRp yuav nyob ntawm ntau lub tswv yim hauv cov kab mob.
4-Nitrophenyl-N-acetyl-β- D-glucosaminide yog ib qho tseem ceeb substrate rau ceev colorimetric assay ntawm N-acetyl-b-glucosaminidase kev ua nyob rau hauv tib neeg cov zis.Chromogenic β-Glucosaminidase substrate yielding cov tshuaj daj thaum cleavage, ua haujlwm tshwj xeeb hauv cov poov xab thiab pwm.
