Cov khoom

EAAT2 glutamate transporter, suav txog> 90% ntawm hippocampal glutamate uptake.Txawm hais tias EAAT2 feem ntau qhia hauv astrocytes, ∼ 10% ntawm EAAT2 molecules muaj nyob rau hauv axon terminals.Txawm hais tias qib qis ntawm EAAT2 qhia hauv glutamatergic terminals, thaum hippocampal slices yog incubated nrog tsawg concentration ntawm d-aspartate (ib qho EAAT2 substrate), axon terminals accumulate d-aspartate sai li astroglia.Qhov no txhais tau hais tias qhov tsis sib haum xeeb ntawm qhov kev faib tawm ntawm EAAT2 protein thiab EAAT2-kev ua haujlwm thauj mus los.Ib qho kev xav yog tias (1) heteroexchange ntawm sab hauv substrate nrog lwm substrate yog considerably sai tshaj li net uptake thiab (2) terminals nyiam heteroexchange vim hais tias ntawm cov qib siab ntawm internal glutamate.Txawm li cas los xij, tam sim no tsis paub meej tias heteroexchange thiab uptake muaj qhov zoo sib xws lossis sib txawv.Txhawm rau daws qhov teeb meem no, peb tau siv qhov kev tsim kho tshiab los sib piv cov txheeb ze ntawm ob txheej txheem hauv nas thiab nas.Net uptake yog sensitive rau cov kev hloov nyob rau hauv daim nyias nyias muaj peev xwm thiab tau stimulated los ntawm sab nraud permeable anions nyob rau hauv kev pom zoo nrog lub hav zoov ntawm uncoupled anion conductance.Los ntawm kev siv cov tom kawg, peb kuj qhia tau tias tus nqi ntawm heteroexchange kuj nyob ntawm lub peev xwm ntawm daim nyias nyias.Tsis tas li ntawd, peb cov ntaub ntawv ntxiv qhia tias muaj cov dej xau sodium hauv EAAT2.Los ntawm kev koom nrog cov kev tshawb pom tshiab hauv peb cov qauv yav dhau los ntawm glutamate uptake los ntawm EAAT2, peb kwv yees tias qhov hluav taws xob rhiab heev ntawm kev sib pauv yog tshwm sim los ntawm qhov voltage-dependent thib peb Na(+) khi.Tsis tas li ntawd, ob qho tib si peb cov kev sim thiab simulations qhia tias tus txheeb ze tus nqi ntawm cov nyiaj tau txais txiaj ntsig thiab heteroexchange yog sib piv hauv EAAT2.