Cov khoom

Adenosine yog ib qho endogenous regulator ntawm vascular laus.Qhov kev ua haujlwm ntawm adenosine no raug txiav tawm los ntawm nws qhov nce thiab cov metabolism hauv microvascular endothelial hlwb (MVEC).Cov khoom thauj tseem ceeb tshaj plaws yog ENT1 (equilibrative nucleoside transporter subtype 1).MVEC kuj qhia txog nucleobase transporter (ENBT1) uas koom nrog hauv cellular flux ntawm adenosine metabolites xws li hypoxanthine.Kev hloov pauv hauv ib qho ntawm cov kev thauj mus los no yuav cuam tshuam rau bioactivity ntawm adenosine thiab nws cov metabolism, suav nrog kev tsim cov pa oxygen dawb radicals.MVEC cais tawm ntawm cov leeg pob txha ntawm ENT1 (+/+) thiab ENT1 (-/-) nas tau raug oxidative kev nyuaj siab vim yog simulated ischemia / reperfusion lossis menadione.Cov dej num ua haujlwm ntawm ENT1 thiab ENBT1 tau raug soj ntsuam raws li xoom-trans influx kinetics ntawm radiolabeled substrates.Muaj qhov txo qis ntawm ENBT1-mediated hypoxanthine uptake los ntawm ENT1 (+/+) MVEC kho nrog menadione lossis tom qab raug rau cov xwm txheej uas simulate isc hemia / reperfusion.Hauv ob qho tib si, superoxide dismutase mimetic MnTMPyP txo qis kev ua haujlwm ntawm ENBT1, cuam tshuam cov superoxide radicals hauv cov lus teb.Hauv qhov sib piv, MVEC cais tawm los ntawm ENT1(-/-) nas pom tsis muaj kev txo qis hauv ENBT1 kev ua haujlwm thaum kho nrog menadione lossis simulated ischemia / reperfusion, tab sis lawv tau muaj cov kev ua haujlwm catalase ntau dua piv rau ENT1(+/+) MVEC.Cov ntaub ntawv no qhia tias ENBT1 kev ua haujlwm tau txo qis hauv MVEC hauv kev teb rau qhov nce superoxide radical uas cuam tshuam nrog ischemia / reperfusion raug mob.MVEC cais tawm los ntawm ENT1(-/-) nas tsis qhia qhov txo qis hauv ENBT1, tejzaum nws yog vim muaj kev ua haujlwm catalase.