Cov khoom

Hauv daim ntawv no, pH-inductive protein-scaffold biosynthesis ntawm cov duab-tunable crystalline kub nanoparticles tau tsim nyob rau hauv chav tsev kub.Los ntawm kev siv yooj yim ntawm cov tshuaj tiv thaiv pH, anisotropic kub nanoparticles suav nrog spheres, daim duab peb sab thiab lub voos xwmfab tuaj yeem tsim los ntawm incubating aqueous tov ntawm sodium tetrachloroaurate nrog Dolichomitriopsis diversiformis biomasses tom qab muab tso rau hauv ultrapure Millipore dej thaum hmo ntuj.Moss protein nrog qhov hnyav ntawm molecular txog 71 kDa thiab pI ntawm 4.9 yog thawj biomolecule koom nrog hauv biosynthesis ntawm kub nanoparticles.Qhov thib ob configuration ntawm cov proteins los ntawm CD spectrum implied hais tias cov moss protein yuav tso saib txawv theem ob configurations xws li random coil, α-helix thiab intermediate conformations ntawm random coil thiab α-helix rau kev sim pH tov.Cov txheej txheem kev loj hlob ntawm kub nanoparticles ntxiv qhia tau hais tias moss protein ntau configurations muab lub template sc affold rau cov duab-tswj biosynthesis ntawm kub nanoparticles.Txawm li cas los xij, qhov kev txwv ntawm cov kub nanoparticles, tau ploj mus hauv boiled moss extract.Cov kub nanoparticles nrog tsim morphology tau ntse reconstructed siv moss protein purified los ntawm kub nanoparticles.Cov yam ntxwv ntawm tus yam ntxwv los ntawm SEM, TEM thiab SAED tau pom tias daim duab peb sab thiab kub kub nanoparticles yog ib qho crystalline.