Cov khoom

Bicine yog ib tug zwitterionic amino acid tsis, nquag nyob rau hauv pH 7.6-9.0 ntau yam (pKa ntawm 8.26 ntawm 25 ° C).Pom zoo tsis rau qhov kub thiab txias ua haujlwm biochemical.Bicine yog siv rau kev npaj cov tshuaj ruaj khov substrate rau kev txiav txim siab serum guanase.Kev siv bicine nyob rau hauv ib txheej nyias ion pauv chromatography txoj kev rau cov protein daws tau raug luam tawm.Bicine tau siv rau hauv peptide thiab protein crystallization.Kev kawm kinetic ntawm quaternary hloov pauv-xeev analogue complex ntawm creatine kinase siv bicine hauv cov tshuaj tiv thaiv tsis zoo.Multiphasic buffer system rau SDS-PAGE ntawm cov protein thiab peptides uas suav nrog bicine tau piav qhia.

Ib leeg alveolar phab ntsa raug rau qhov ntev-nrhiav kev kawm nyob rau hauv saline thiab Bicine (0.2 M) mus rau ib tug zuj zus decay nyob rau hauv cov ntaub so ntswg nro (TTD).Peb tau tshuaj xyuas cov txiaj ntsig ntawm cov kev daws teeb meem sib txawv ntawm TTD no thiab nrhiav kev hloov pauv hauv cov txheej txheem ultrastructure.Lub ntsws parenchyma tau muab faib rau ib leeg alveolar phab ntsa (30 X 30 X 150 microns) hauv phosphate-buffered saline (0.15 M).Hloov mus rau qhov ntev-nruj da dej, cov ntaub so ntswg tau muab tso rau hauv Bicine, saline, fortified Hank cov tshuaj, 0.25% Alcian xiav hauv saline, los yog sodium dodecyl sulfate tov, rau lub sij hawm sib txawv.Cycled los ntawm qhov muab txuas ntxiv nrog lub zog siab tshaj plaws ntsuas lub sijhawm, cov ntaub so ntswg tib yam tau kho nyob rau hauv buffered glutaraldehyde / tannic acid thiab ua tiav rau electron microscopy.Ib phab ntsa alveolar immersed nyob rau hauv saline los yog Bicine pom ib tug zuj zus TTD.Vacuoles los yog qhov chaw tau tshwm sim nyob rau hauv interstitium uas nrog cellular disorganization zuj zus nrog TTD.Pom tsis pub dhau 0.3 teev, cov kev hloov pauv tau zoo tshaj ntawm 0.6 teev.Hauv sodium dodecyl sulfate (70 mM), txawm li cas los xij, tsis muaj TTD thiab cov txheej txheem tsis muaj interstitium, tsuas yog hauv qab daus daim nyias nyias thiab fibrous proteins.Nyob rau hauv fortified Hank cov tshuaj los yog 0.25% Alcian xiav lub interstitial matrix, cell morphology thiab cov ntaub so ntswg nro tau zoo khaws cia rau 1 h.Txoj kev tshawb no qhia tias leaching ntawm interstitial matrix tshwm sim nyob rau hauv saline los yog Bicine, thiab ib tug zoo matrix yog ib qho tseem ceeb rau kev khaws cia ntawm cov ntaub so ntswg nro.