Cov khoom

Uropathogenic hom Escherichia coli (UPEC) yog qhov ua rau muaj kab mob urinary kab mob.Kev ciaj sia nyob rau hauv cov hlab ntsha yog txuam nrog cov txheej txheem sib txawv uas pab tiv thaiv cov ntshav qabzib sib txuas-kho kom sib haum.Thaum cov phenotypic heterogeneity ntawm cov kab mob tau pom tias muaj kev cuam tshuam rau cov tshuaj tua kab mob, qhov ua tau tias nws ua rau cov cell refractory tua los ntawm lub cev tiv thaiv kab mob tsis tau sim sim.Nyob rau hauv txoj kev tshawb no peb nrhiav los txiav txim seb qhov heterogeneity ntawm kab lis kev cai kab lis kev cai muaj feem xyuam rau cov kab mob hom phiaj los ntawm cov ntshav qab zib.Peb tau saib xyuas cov cell faib hauv UPEC hom CFT073 nrog cov fluorescent reporter protein.Cov hlwb nyob ruaj ruaj tau raug tsim nyob rau hauv lub cev ua haujlwm lossis kub tsis ua haujlwm rau tib neeg cov ntshav nyob rau hauv lub xub ntiag lossis tsis muaj cov tshuaj tua kab mob sib txawv (ampicillin, norfloxacin, thiab amikacin), thiab kev faib tawm ntawm tes thiab ntxiv cov protein C3 khi tau ntsuas los ntawm kev ntws cytometry thiab immunofluorescence microscopy.Heterogeneity nyob rau hauv ob npaug ntawm lub sij hawm ntawm CFT073 hlwb nyob rau hauv cov ntshav tau enabled peb phenotypically sib txawv subpopulations kom paub qhov txawv, tag nrho cov ntawm lawv tau lees paub los ntawm C3 tivthaiv ntawm lub complement system.Cov pej xeem ntawm cov cell loj hlob sai heev tiv thaiv cov ntshav qab zib-kuaj kho lysis.Qhov tseem ceeb subpopulation ntawm cov hlwb nrog nruab nrab kev loj hlob tus nqi yog susceptible rau serum.Cov neeg thib peb, uas tsis rov pib qhov kev loj hlob raws li qhov dilution los ntawm qhov chaw nyob ruaj khov, yog ib txhij tiv thaiv los ntawm cov ntshav ntxiv thiab tshuaj tua kab mob.