Ammonium hexachloroiridate (IV) CAS: 16940-92-4
| Catalog Number | xwm 90606 |
| Khoom npe | Ammonium hexachloroiridate (IV) |
| CAS | 16940-92-4 ib |
| Molecular Formula | H8Cl6IrN2 |
| Molecular Luj | 441.012 ib |
| Cov ntaub ntawv khaws cia | Ambient |
Khoom Specification
| Qhov tshwm sim | Olive ntsuab crystals |
| Assay | 99% |
Oxidative puas tsuaj rau DNA hauv paus feem ntau tshwm sim los ntawm kev tsim cov oxidized purines, tshwj xeeb tshaj yog 7,8-dihydro-8-oxoguanine (8-oxoG) thiab 7,8-dihydro-8-oxoadenine (8-oxoA), yav tas los ua ib tug zoo- paub mutagenic lesion.Txij li thaum 8-oxoG tau nkag mus rau oxidation ntxiv piv nrog cov hauv paus ib txwm, qhov ntxig ntawm lub hauv paus thaum lub sij hawm DNA synthesis tawm tsam ib daim ntawv oxidized ntawm 8-oxoG tau tshawb xyuas hauv vitro.Ib qho qauv hluavtaws uas muaj ib qho 8-oxoG qhov txhab raug kho thawj zaug nrog sib txawv ib-electron oxidants los yog nyob rau hauv singlet oxygen tej yam kev mob thiab ces raug rau primer extension catalyzed los ntawm Klenow fragment exo- (Kf exo-), calf thymus DNA polymerase alpha (pol alpha. ) lossis human DNA polymerase beta (pol beta).Raws li cov lus ceeb toom dhau los, dAMP thiab dCMP tau muab tso rau hauv kev xaiv rov qab 8-oxoG nrog tag nrho peb DNA polymerases.Interestingly, oxidation ntawm 8-oxoG tau pom los induce dAMP thiab dGMP insertion opposite lub qhov txhab los ntawm Kf exo- nrog ib ntus inhibition ntawm primer extension o tshwm sim ntawm qhov chaw ntawm lub hauv paus hloov.Tsis tas li ntawd, qhov txhab ua ib qho thaiv thaum lub sij hawm DNA synthesis los ntawm pol alpha thiab pol beta.Kev sim nrog 8-oxoA-hloov template oligonucleotide qhia tias ob qho tib si 8-oxoA thiab oxidized daim ntawv ntawm 8-oxoA ncaj qha ntxig ntawm dTMP los ntawm Kf exo-.Mass spectrometric tsom xam ntawm 8-oxoG-muaj oligonucleotides ua ntej thiab tom qab oxidation nrog IrCl62-yog zoo ib yam nrog oxidation ntawm feem ntau 8-oxoG site, uas ua rau tsim ib tug guanidinohydantoin moiety raws li cov khoom loj.Tsis muaj pov thawj los tsim cov chaw abasic tau txais.Cov txiaj ntsig no qhia tau tias daim ntawv oxidized ntawm 8-oxoG, tejzaum nws yog guanidinohydantoin, tuaj yeem coj kev nyeem ntawv tsis raug thiab tsis raug ntawm dNTPs thaum lub sijhawm DNA synthesis.Yog tias cov txheej txheem zoo li no tshwm sim hauv vivo, nws yuav sawv cev rau qhov tshwm sim mutagenic lesion ua rau G--> T thiab G--> C hloov pauv.Txawm li cas los xij, daim ntawv oxidized sib thooj ntawm 8-oxoA feem ntau qhia qhov tseeb ntawm T thaum lub sij hawm DNA synthesis nrog Kf exo-.
