Cov khoom

Ib qho kev ua tau zoo aptasensor tau tsim nyob rau hauv uas graphene oxide (GO) tau ua haujlwm los ua qhov taw qhia rau ob qho tib si electrochemical impedance spectroscopy thiab electrochemiluminescence (ECL) teeb liab tiam.Lub aptasensor tau tsim los ntawm nws tus kheej sib sau ua ke ECL sojntsuam ntawm thiolated adenosine triphosphate binding aptamer (ABA) tagged nrog Ru complex (Ru (bpy) 3 (2+) derivatives) rau saum npoo ntawm kub nanoparticle (AuNP) hloov glassy carbon electrode (GCE).ABA immobilized mus rau AuNP hloov GCE tuaj yeem ua rau adsorb GO vim muaj zog π-π kev sib cuam tshuam ntawm ABA thiab graphene oxide;ECL quenching ntawm Ru complex yuav siv sij hawm qhov chaw vim lub zog hloov thiab electron hloov, thiab ib tug loj nce ntawm lub electron hloov tsis kam (Ret) ntawm lub electrode.Thaum nyob rau hauv lub xub ntiag ntawm lub hom phiaj adenosine triphosphate (ATP), ABA nyiam tsim ABA-ATP bioaffinity complexes, uas tsis muaj zog affinity rau graphene oxide thiab khaws cov graphene oxide deb ntawm electrode nto, yog li tso cai rau ECL teeb liab txhim kho, thiab nrog rau qhov txo qis ntawm Ret.Vim hais tias ntawm ECL quenching efficiency siab, cov qauv tshwj xeeb, thiab cov khoom siv hluav taws xob ntawm graphene oxide, Ret thiab ECL siv piv rau cov logarithm ntawm ATP concentration yog linear nyob rau hauv ntau yam ntawm 10 pM mus rau 10 nM nrog ib tug ultra-tsawg nrhiav pom txwv ntawm 6.7 pM rau 4.8 pM.Lub tswv yim aptasensor tau nthuav tawm zoo heev reproducibility, stability, thiab zoo heev xaiv, thiab ATP yuav ua tau zoo txawv ntawm nws cov analogues.Qhov tseem ceeb tshaj, qhov kev ua tau zoo ECL aptasensor lub tswv yim no raws li GO ua ob qho tib si raws li cov khoom siv hluav taws xob thiab ECL cov teeb liab qhia tau dav dav thiab tuaj yeem txuas tau yooj yim mus rau lwm cov xwm txheej lom neeg.