PII teeb liab processor proteins yog dav dav nyob rau hauv prokaryotes thiab cov nroj tsuag uas lawv tswj ib tug ntau ntawm cov tshuaj tiv thaiv anabolic.Ua tau zoo tshaj ntawm cov metabolites yuav tsum tau so kom nruj cellular tswj circuits.Ntawm no peb qhia tau hais tias ib qho kev hloov pauv hauv PII signaling protein los ntawm cyanobacterium Synechocystis sp.PCC 6803 txaus los qhib txoj hauv kev arginine ua rau ntau dhau ntawm biopolymer cyanophycin (multi-L-arginyl-poly-L-aspartate).Cov khoom no yog kev txaus siab rau biotechnological raws li qhov chaw ntawm cov amino acids thiab polyaspartic acid.Txoj haujlwm no ua piv txwv txog txoj hauv kev tshiab ntawm txoj hauv kev engineering los ntawm kev tsim cov kev cai tsim PII signaling proteins.Ntawm no, tus engineered Synechocystis sp.PCC6803 strain nrog PII-I86N kev hloov pauv ntau tshaj-accumulated arginine los ntawm kev ua haujlwm ntawm cov enzyme tseem ceeb N-acetylglutamate kinase (NAGK).In the engineered strain BW86, in vivo NAGK kev ua tau zoo nce thiab ua rau ntau tshaj kaum npaug ntawm cov ntsiab lus siab dua argin. tshaj nyob rau hauv qus-hom.Raws li qhov tshwm sim, cov kab mob BW86 tau nce mus txog 57% cyanophycin ib lub xov tooj ntawm tes qhuav nyob rau hauv cov xwm txheej sim, uas yog cov txiaj ntsig siab tshaj plaws ntawm cyanophycin tshaj tawm rau hnub tim.Strain BW86 tsim cyanophycin nyob rau hauv ib qho molecular mass ntau ntawm 25 txog> 100 kDa;Cov tsiaj qus-hom tsim cov polymer nyob rau hauv ib thaj tsam ntawm 30 mus rau> 100 kDa.Qhov siab yield thiab siab molecular loj ntawm cyanophycin tsim los ntawm strain BW86 nrog rau cov uas tsis muaj as-ham uas yuav tsum tau ntawm cyanobacteria ua rau nws ib tug cog lus txhais tau tias rau biotechnological zus tau tej cov cyanophycin.Txoj kev tshawb no ntxiv qhia txog qhov ua tau ntawm cov txheej txheem metabolic siv cov PII signaling protein, uas tshwm sim hauv ntau cov kab mob.
