PIPES Cas: 5625-37-6 Dawb crystalline hmoov 99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE
| Catalog Number | xwm 90117 |
| Khoom npe | PIPES (Piperazine-1,4-bis (2-ethanesulfonic acid)) |
| CAS | 5625-37-6 |
| Molecular Formula | C8H18N2O6S2 |
| Molecular Luj | 302.37 Nws |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 2933599 ib |
Khoom Specification
| Cov hlau hnyav | <5 ppm |
| Poob rau ziab | <1.0% |
| Solubility | Clear, Colorless tov (5% 1N NaOH) |
| Assay | 99 - 101% |
| Qhov tshwm sim | Dawb crystalline hmoov |
PIPES [piperazine-N, N′-bis (2-ethanesulfonic acid)] feem ntau yog siv los ua tus neeg sawv cev buffering hauv biochemistry.Nws yog ib qho ethanesulfonic acid tsis tsim los ntawm Good et al.hauv 1960s.PIPES muaj pKa ze ntawm lub cev pH uas ua rau nws muaj txiaj ntsig zoo hauv kev ua haujlwm ntawm tes.Nws tau raug sau tseg kom txo qis lipid poob thaum buffering glutaraldehyde histology hauv cov nroj tsuag thiab tsiaj cov ntaub so ntswg.Ib tus neeg sawv cev nrog pKa ze ntawm lub cev pH.
Antimicrobial peptide magainin 2 tsim pores hauv lipid daim nyias nyias thiab induces membrane permeation ntawm cellular txheem.Txawm hais tias qhov permeation no yuav yog qhov ua rau tseem ceeb ntawm nws cov kab mob bactericidal, cov txheej txheem ntawm pore tsim tseem tsis to taub.Yog li ntawd, peb tau tshawb xyuas qhov nthuav dav ntawm kev sib cuam tshuam ntawm magainin 2 nrog lipid daim nyias nyias siv ib qho loj heev unilamellar vesicles (GUVs).Kev khi ntawm magainin 2 mus rau lipid daim nyias nyias ntawm GUVs tau nce qhov kev hloov pauv ntawm thaj tsam ntawm daim nyias nyias, δ, uas yog proportional rau qhov concentration ntawm magainin 2, X. Qhov no qhia tau hais tias tus nqi tas li ntawm magainin 2-induced ob. -state kev hloov pauv ntawm lub xeev tsis zoo mus rau lub xeev pore zoo heev nrog kev nce hauv δ.Qhov nro ntawm lipid daim nyias nyias tom qab aspiration ntawm GUV kuj tau qhib magainin 2-induced pore tsim.Txhawm rau nthuav tawm qhov chaw ntawm magainin 2, kev sib cuam tshuam ntawm carboxyfluorescein (CF)-labeled magainin 2 (CF-magainin 2) nrog ib qho GUVs uas muaj cov dej-soluble fluorescent sojntsuam, AF647, tau tshawb xyuas siv confocal microscopy.Thaum tsis muaj qhov nro vim kev ntshaw, tom qab kev sib cuam tshuam ntawm magainin 2 qhov kev siv fluorescence ntawm GUV rim vim CF-magainin 2 tau nce sai heev rau tus nqi ruaj khov, uas nyob twj ywm rau lub sijhawm ntev, thiab ntawm 4-32 s ua ntej. qhov pib ntawm kev xau ntawm AF647 qhov kev siv lub npoo pib nce nrawm mus rau lwm tus nqi ruaj khov.Nyob rau hauv sib piv, nyob rau hauv lub xub ntiag ntawm qhov nro, tsis muaj kev nce nyob rau hauv rim siv ua ntej pib ntawm to.Cov txiaj ntsig no qhia tau tias magainin 2 tsis tuaj yeem hloov pauv los ntawm sab nrauv mus rau sab hauv monolayer kom txog thaum ua ntej pore tsim.Raws li cov txiaj ntsig no, peb txiav txim siab tias magainin 2-induced pore yog ib qho stretch-activated pore thiab stretch ntawm sab hauv monolayer yog lub zog tsav ntawm lub pore tsim.
