ONPG CAS: 369-07-3 98.0% Min Dawb Los Tawm -White Powder
| Catalog Number | XD90006 |
| CAS | 369-07-3 : kuv |
| Khoom npe | ONPG (2-Nitrophenyl-beta-D-galactopyranoside) |
| Molecular Formula | C12H15NO8 |
| Molecular Luj | 301.25 Nws |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
| Harmonized tariff Code | 29 400 000 IB |
Khoom Specification
| Purity (HPLC) | Min.98.0% |
| Qhov tshwm sim | Dawb rau tawm - dawb hmoov |
| Kev daws(1% hauv dej) | Ntshiab, tsis muaj xim mus rau xim daj me ntsis |
| Cov ntsiab lus dej(Karl Fisher) | Max.0.5% |
| Kev sib hloov kho qhov muag tshwj xeeb [α]D20(c=1, H2O) | -65.0 ° C txog -73.0 ° C |
Kev sib tham ntawm ONPG test (β-galactosidase test)
Cov lus nug feem ntau tau hais tsis ntev los no: 1. Vim li cas ONPG tuaj yeem siv los txheeb xyuas qhov qeeb lactose fermentation?2. Vim li cas lub teb chaws tus qauv hais tias nws yuav tsum tau siv 3% sodium chloride trisaccharide hlau (los yog trisaccharide hlau) rau ONPG xeem?3. Rau Vibrio parahaemolyticus, thaum ua OPNG xeem, vim li cas yuav tsum tau ntxiv toluene dropwise raws li tus qauv?Lub luag haujlwm yog dab tsi?
Peb lub tuam txhab tau tshuaj xyuas ntau cov ntaub ntawv, sau cov ntsiab lus, thiab qhia rau koj hauv qab no:
Lub hauv paus ntsiab lus: Suav lub npe ntawm ONPG yog o-nitrobenzene-β-D-galactopyranoside.ONPG tuaj yeem hydrolyzed rau hauv galactose thiab daj o-nitrophenol (ONP) los ntawm β-galactosidase, yog li kev ua haujlwm ntawm β-galactosidase tuaj yeem kuaj pom los ntawm kev hloov xim ntawm kab lis kev cai nruab nrab.
Lactose yog cov piam thaj uas feem ntau cov kab mob yuav tsum tau kuaj xyuas.Nws cov metabolism yuav tsum muaj ob lub enzymes, ib qho yog cell permease, lactose nkag mus rau hauv hlwb raws li kev ua ntawm permease;Lwm qhov yog β-galactosidase, uas hydrolyzes lactose rau hauv galactose.Lactose thiab qabzib.β-Galactosidase tseem tuaj yeem ua ncaj qha rau ONPG kom hydrolyze nws mus rau hauv galactose thiab daj o-nitrophenol (ONP).Nws tuaj yeem ua tiav hauv 24 teev, txawm tias muaj lactose qeeb fermenters.Yog li, nws piav qhia cov txiaj ntsig ntawm kev xaiv kab lis kev cai 1 los ntawm agar slant thiab inoculating nws nyob rau hauv tag nrho lub voj voog ntawm 36 ° C rau 1-3h thiab 24h.Yog tias β-galactosidase raug tsim, nws yuav tig daj hauv 1-3h, yog tias tsis muaj cov enzyme no, nws yuav tsis hloov xim hauv 24h.
Raws li cov enzymes saum toj no, cov kab mob tuaj yeem muab faib ua pawg hauv qab no:
1 lactose-fermenting (18-24 teev) cov kab mob nrog permease thiab β-galactosidase P + G +;
2 Ncua lactose fermenters (siv ntev dua 24 teev) tsis muaj permease tab sis muaj galactosidase: P-G +.
3 Tsis-lactose fermenters tsis muaj permease thiab galactosidase: P-G-.
Kev kuaj ONPG tuaj yeem siv los paub qhov txawv ntawm cov kab mob lactose-lag-fermenting (P-G +) los ntawm cov kab mob uas tsis yog-fermenting lactose (PG-), xws li:
1 Sib txawv lig lactose fermenters (P-G +) los ntawm cov tsis-lactose fermenters (P- G-).
(a) Citrobacter (+) thiab Salmonella arizonae (+) los ntawm Salmonella (-).
(b) Escherichia coli (+) los ntawm Shigella sonnei (-).
Vim li cas ONPG kev soj ntsuam tau ua los ntawm kev siv cov kab lis kev cai thaum hmo ntuj ntawm 3% sodium chloride ferric trisaccharide (hlau trisaccharide)?Peb lub tuam txhab tau sab laj ntau cov ntaub ntawv, tab sis tsis muaj cov lus qhia meej.Tsuas yog nyob rau ntawm FDA lub vev xaib, nws tau sau tias "Inoculate kab lis kev cai yuav tsum tau sim mus rau triple qab zib hlau agar slants thiab incubate rau 18 h ntawm 37 ° C (los yog lwm yam tsim nyog kub, yog tias xav tau). Nutrient (los yog lwm yam) agar slants muaj 1.0 % lactose kuj tseem siv tau. "txhais tau tias: cov kab mob kuaj tau raug inoculated ntawm trisaccharide hlau nruab nrab thiab kab lis kev cai ntawm 37 ° C rau 18h.Khoom noj khoom haus agar slants (los yog lwm yam) nruab nrab uas muaj 1% lactose kuj tau txais.Yog li, nws yog inferred tias trisaccharide hlau nruab nrab muaj lactose.Tom qab kev loj hlob ib hmos, cov kab mob tau tsim ib qho zoo active β-galactosidase.Siv cov kab mob no, ONPG tuaj yeem decomposed los ntawm β-galactosidase sai dua.Qhov kev sim tshwm sim sai dua thiab zoo dua tshwm sim.Tsis tas li ntawd, dropwise ntxiv ntawm toluene thiab da dej rau 5 min yog tag nrho kom tso siab tso tawm β-galactosidase.
