Cov khoom

1.Metabolite profiling txoj kev yog cov cuab yeej tseem ceeb rau kev ntsuas cov metabolite pas dej hauv cov kab mob lom.Txawm hais tias feem ntau cov txheej txheem metabolite profileing qhia txog kev sib txheeb los yog nyob ntawm ob peb tus qauv sab hauv uas sawv cev rau tag nrho cov metabolites, qhov kawg yuav tsum tau ua kom muaj nuj nqis piav qhia ntawm cov pas dej metabolite hauv cov hlwb thiab cov kua dej yog qhov kev txiav txim siab meej.Peb tshaj tawm ntawm no ib tug high-throughput thiab rhiab roj chromatography / tandem huab hwm coj spectrometry (GC / MS / MS) tsom metabolite profiling txoj kev ua kom muaj nuj nqis ntawm tag nrho cov metabolites pom.Cov txheej txheem yog ua raws li methyl chloroformate derivatization thiab quantification los ntawm spiking cov qauv nrog cov qauv metabolite cais derivatized nrog deuterated derivatization reagents.Cov tsoos hluav taws xob cuam tshuam ionization yog hloov nrog cov tshuaj ionization zoo txij li tom kawg mus rau qhov loj dua khaws cov molecular ion thiab lwm cov khoom hnyav molecular.Qhov no ua rau nws ea sier xaiv tshwj xeeb MS / MS kev hloov pauv ntawm ntau cov coeluting metabolites.Tam sim no, cov txheej txheem tshiab GC / MS / MS muaj 67 hom metabolites uas feem ntau muaj nyob hauv pawg amino thiab nonamino organic acids.Peb qhia txog qhov siv tau ntawm cov qauv ntawm cov zis thiab cov ntshav kuaj.Cov txheej txheem yog ib qho kev txhim kho tseem ceeb ntawm cov txheej txheem tam sim no rau ntau GC / MS metabolite profileing ntawm amino acids thiab nonamino organic acids.

2.24S-Hydroxycholesterol (24OHC) thiab 27-hydroxycholesterol (27OHC) yog ob yam zoo sib xws oxysterols ntawm txawv keeb kwm - lub qub yuav luag tsim nyob rau hauv lub hlwb thiab lub tom kawg tsim rau ib tug tsawg npaum li cas nyob rau hauv lub hlwb tshaj nyob rau hauv feem ntau lwm yam kabmob.HYPOTHESIS YUAV TSUM: Neuronal puas thiab/los yog demyelination ua rau muaj zog flux ntawm 24OHC los ntawm lub hlwb mus rau hauv lub cerebrospinal kua (CSF), whereas ib tug tsis xws luag ntshav-hlwb barrier ua rau muaj zog flux ntawm 27OHC los ntawm cov kev mus rau hauv lub CSF.Isotope dilution-mass spectrometry tau siv los soj ntsuam ob lub oxysterols hauv CSF thiab ntshav ntshav los ntawm ntau dua 250 cov neeg mob uas muaj cov kab mob sib txawv ntawm cov hlab ntsha thiab cov kab mob geriatric.Cov CSF-theem ntawm ob lub oxysterols tau cuam tshuam ntau dua los ntawm cov kab mob sib txawv dua li qib ntshav plasma.Cov neeg mob uas muaj cov kab mob demyelinating nquag tau nce qib ntawm 24OHC hauv CSF nrog qhov sib piv ntawm 24OHC / 27OHC.Cov neeg mob uas muaj mob meningitis feem ntau muaj qib siab ntawm ob qho steroids nrog qis 24OHC / 27OHC piv.Cov neeg mob Alzheimer's tus kab mob tau nce me ntsis ntawm 24OHC hauv CSF nrog tsawg dua hauv 27OHC.Cov neeg mob uas muaj ntau yam kab mob sclerosis muaj qhov nyiam kom muaj qib siab dua ntawm 24OHC thaum lub sijhawm ua haujlwm nrog qhov siab 24OHC / 27OHC piv. Kev ntsuas ntawm ob lub oxysterols hauv CSF thiab ntshav ntshav yuav ntxiv qhov tseem ceeb rau cov txheej txheem biochemical uas twb muaj lawm rau kev ntsuam xyuas cov kab mob neurological.