Dithizone CAS: 60-10-6 80% Dub xiav hmoov
| Catalog Number | xwm 90464 |
| Khoom npe | Dithizone |
| CAS | 60-10-6 ib |
| Molecular Formula | C13H12N4S |
| Molecular Luj | 256.33 Nws |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 29 3098 ib |
Khoom Specification
| Poob rau ziab | ≤ 5% |
| Qhov tshwm sim | Dub hmoov hmoov |
| Assay | 80% |
| Residue ntawm Ignition | ≤ 0.2% |
Mesenchymal qia hlwb (MSCs) tau raug tshaj tawm tias yog qhov chaw txaus nyiam rau tiam neeg ntawm cov kab mob hloov pauv tau β hlwb.Ib tug murine embryonic mesenchymal progenitor cell kab C3H10T1/2 tau raug lees paub tias yog tus qauv rau MSCs, vim tias nws muaj peev xwm sib txawv ntawm ntau haiv neeg.Lub hom phiaj ntawm txoj kev tshawb no yog tshawb xyuas seb C3H / 10T1 / 2 hlwb puas muaj peev xwm los sib txawv rau hauv cov hlwb tsim cov tshuaj insulin (IPCs).Ntawm no, peb tau tshawb xyuas thiab muab piv rau qhov sib txawv hauv vitro ntawm nas MSCs thiab C3H10T1/2 hlwb rau hauv IPCs.Tom qab cov hlwb tau txais kev sib txawv ntawm IPC, qhov kev qhia ntawm cov cim sib txawv tau kuaj pom los ntawm immunocytochemistry, thim rov qab transcription-polymerase saw cov tshuaj tiv thaiv (RT-PCR), ntau npaum li cas ntawm RT-PCR (qRT-PCR) thiab Western blotting.Cov tshuaj insulin secretion tau soj ntsuam los ntawm enzyme-linked immunosorbent assay (ELISA).Tsis tas li ntawd, cov hlwb sib txawv no tau hloov mus rau hauv streptozotocin-induced ntshav qab zib nas thiab lawv cov kev ua haujlwm lom neeg tau raug kuaj hauv vivo.Txoj kev tshawb no qhia txog 2-theem txoj hauv kev los tsim IPCs los ntawm C3H10T1/2 hlwb.Raws li cov xwm txheej tshwj xeeb induction rau 7-8 hnub, C3H10T1 / 2 hlwb tsim peb-dimensional spheroid lub cev (SBs) thiab secreted insulin, thaum tiam IPCs tau los ntawm nas MSCs yuav tsum tau ntev (ntau tshaj 2 lub lis piam).Tsis tas li ntawd, cov IPCs no tau muab los ntawm C3H10T1/2 hlwb tau txhaj rau hauv cov nas mob ntshav qab zib thiab txhim kho cov piam thaj hauv qab, lub cev hnyav thiab ua rau pom cov piam thaj ib txwm muaj.Txoj kev tshawb fawb tam sim no tau muab ib qho yooj yim thiab ncaj ncees hauv vitro qauv rau kev tshawb xyuas ntxiv txog cov txheej txheem hauv qab IPC qhov sib txawv ntawm MSCs thiab kev kho cell hloov kho rau ntshav qab zib.
