dCMP, 2'-Deoxycytidine 5'-monophosphate, dawb acid CAS: 1032-65-1
| Catalog Number | xwm 90549 |
| Khoom npe | dCMP, 2'-Deoxycytidine 5'-monophosphate, dawb acid |
| CAS | 1032-65-1 : kuv |
| Molecular Formula | C9H14N3O7p |
| Molecular Luj | 307.20 Nws |
| Cov ntaub ntawv khaws cia | -15 rau -20 ° C |
| Harmonized tariff Code | 293499 0 |
Khoom Specification
| Qhov tshwm sim | Khoom |
| Assay | 99% |
Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) yog ib qho muaj zog tshwj xeeb inhibitor ntawm tus kab mob siab C (HCV) RNA synthesis hauv Huh-7 relicon cells.Txhawm rau tiv thaiv HCV NS5B RNA polymerase, PSI-6130 yuav tsum tau phosphorylated rau daim ntawv 5'-triphosphate.Cov phosphorylation ntawm PSI-6130 thiab inhibition ntawm HCV NS5B tau tshawb xyuas.Lub phosphorylation ntawm PSI-6130 los ntawm recombinant human 2'-deoxycytidine kinase (dCK) thiab uridine-cytidine kinase 1 (UCK-1) tau ntsuas los ntawm kev siv cov tshuaj tiv thaiv spectrophotometric ua ke.PSI-6130 tau pom tias yog ib lub substrate rau purified dCK, nrog ib tug Km ntawm 81 microM thiab ib tug kcat ntawm 0.007 s-1, tab sis tsis yog ib tug substrate rau UCK-1.PSI-6130 monophosphate (PSI-6130-MP) tau zoo phosphorylated rau diphosphate thiab tom qab ntawd rau triphosphate los ntawm tib neeg UMP-CMP kinase thiab nucleoside diphosphate kinase, feem.Qhov inhibition ntawm qus-hom thiab mutated (S282T) HCV NS5B RNA polymerases tau kawm.Qhov ruaj khov-state inhibition tas li (Ki) rau PSI-61 30 triphosphate (PSI-6130-TP) nrog cov enzyme qus yog 4.3 microM.Cov txiaj ntsig zoo sib xws tau txais nrog 2'-C-methyladenosine triphosphate (Ki = 1.5 microM) thiab 2'-C-methylcytidine triphosphate (Ki = 1.6 microM).NS5B nrog kev hloov pauv S282T, uas paub tias yog kev tiv thaiv rau 2'-C-methyladenosine, tau inhibited los ntawm PSI-6130-TP raws li cov tsiaj qus.Kev koom ua ke ntawm PSI-6130-MP rau hauv RNA catalyzed los ntawm purified NS5B RNA polymerase ua rau cov saw txiav.
