Cytidine-5′-triphosphate (CTP), disodium ntsev CAS: 652154-13-7
| Catalog Number | xwm 90551 |
| Khoom npe | Cytidine-5'-triphosphate (CTP), disodium ntsev |
| CAS | 652154-13-7 |
| Molecular Formula | C9H14N3Na2O14P3 |
| Molecular Luj | 527.12 ib |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
Khoom Specification
| Qhov tshwm sim | Dawb hmoov |
| Assay | 99% |
CTP: phosphocholine cytidylyltransferase α (CCTα) yog ib qho enzyme nuclear uas catalyzes tus nqi txwv cov kauj ruam hauv CDP-choline txoj kev rau phosphatidylcholine (PC) synthesis.Lipid activation ntawm CCTα ua rau nws hloov mus rau hauv lub hnab ntawv nuclear thiab nthuav dav ntawm ib qho intranuclear membrane network hu ua nucleoplasmic reticulum (NR) los ntawm cov txheej txheem cuam tshuam nrog cov membrane deformation.Nuclear lamins kuj tseem xav tau rau kev ruaj ntseg thiab kev loj hlob ntawm NR, tab sis txawm tias cov qauv tsim tshwj xeeb no, lossis cov khoom siv nuclear feem ntau, yuav tsum tau ua rau PC synthesis tsis paub.Txhawm rau tshuaj xyuas qhov kev sib raug zoo no, lub tshuab hluav taws xob nuclear tau depleted los ntawm RNAi los yog cuam tshuam los ntawm kev qhia ntawm Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), thiab cov nyhuv ntawm CCTα thiab choline metabolism raug tshuaj xyuas.siRNA-mediated silencing ntawm lamin A / C los yog lamin B1 hauv CHO hlwb kom txo qis NR tsis muaj kev cuam tshuam rau PC synthesis, thaum ob zaug knockdown tsis tshwj xeeb inhibited txoj kev.Kev lees paub t nws lub luag haujlwm me hauv PC synthesis, tsuas yog 10% ntawm transiently overexpressed choline / ethanolamine phosphotransferase tau kuaj pom hauv NR.Hauv CHO hlwb, CCTα yog nucleoplasmic thiab co-localized nrog GFP-progerin hauv nuclear folds thiab invaginations;Txawm li cas los xij, HGPS fibroblasts pom qhov txawv txav ntawm CCTα nyob rau hauv cytoplasm thiab lub hnab ntawv nuclear uas tau nrog los ntawm 2-fold txo hauv PC synthesis.Txawm hais tias nws hloov pauv hauv cheeb tsam, choline-labeling thwmsim tau pom tias CCT kev ua haujlwm tsis cuam tshuam, thiab inhibition ntawm PC synthesis tau taug qab los txo qis kev ua haujlwm ntawm hemicolinium-sensitive choline transporter.Peb xaus lus tias CCTα thiab lamins tshwj xeeb koom tes los tsim NR, tab sis tag nrho cov qauv ntawm lub hnab ntawv nuclear muaj kev cuam tshuam me me rau CCT kev ua thiab PC synthesis.
