Cov khoom

Tshiab zwitterionic butanesulfonic acids uas txuas ntxiv alkaline ntau ntawm plaub tsev neeg ntawm Zoo buffers: kev ntsuam xyuas rau siv nyob rau hauv cov kab mob lom.

Plaub qhov tshiab zwitterionic butanesulfonic acid buffers uas muaj feem cuam tshuam rau plaub tsev neeg ntawm Zoo buffers raug soj ntsuam rau siv hauv cov kab mob lom.Cov buffers no, nrog pKa qhov tseem ceeb ntawm 7.6 txog 10.7, tau muab piv nrog ntau yam ntawm lwm cov buffers los ntawm tib tsev neeg thiab nrog cov tsis muaj feem cuam tshuam los txiav txim lawv cov txiaj ntsig ntawm kev ua enzyme thiab kev loj hlob ntawm microbial.Kev ua haujlwm ntawm plaub enzymes nrog qhov zoo tshaj pH qhov tseem ceeb hauv alkaline ntau yam tau sim: beta-galactosidase, esterase, phosphodiesterase thiab alkaline phosphatase.Feem ntau, tag nrho cov zoo buffers, nrog rau cov tshiab butanesulfonic acid buffers, muab kev ua tau zoo;Txawm li cas los xij, muaj kev hloov pauv hauv kev ua haujlwm ntawm qee cov enzymes nrog qee qhov tsis sib xws.Tris, glycine, thiab phosphate buffers feem ntau pom muaj kev hloov pauv hauv kev ua si piv rau tsev neeg ntawm Good buffers.beta-Galactosidase, tshwj xeeb tshaj yog, pom kev ua haujlwm ntau dua nrog Zoo buffers dua li nrog phosphate lossis Tris buffers.Ib yam li ntawd, kev loj hlob ntawm xya kab mob tau zoo ib yam, nrog rau qee qhov kev zam, rau tag nrho cov tsev neeg Zoo ntawm buffers nrog Tris feem ntau inhibiting kev loj hlob.Quantitation ntawm alkaline phosphatase conjugated rau cov tshuaj tiv thaiv yog ib qho cuab yeej tseem ceeb hauv ntau daim ntawv thov hauv molecular biology.Ntau qhov zoo buffers tau muab cov cim zoo thaum piv nrog Tris ntawm pH 9.5 rau kev tshawb nrhiav cov proteins uas siv cov tshuaj tiv thaiv alkaline phosphatase-conjugated.