Tus tshiab Trinder's reagent yog ib qho dej-soluble aniline derivative uas tau siv dav hauv kev tshuaj ntsuam xyuas thiab kuaj biochemical.Nws muaj ntau qhov zoo dua li cov pa chromogenic reagents hauv kev txiav txim siab colorimetric ntawm hydrogen peroxide kev ua.Tus tshiab Trinder's reagent yog txaus ruaj khov rau ob qho tib si Nws tuaj yeem siv rau hauv kev daws teeb meem lossis hauv kev sim ntsuas cov kav dej.Nyob rau hauv lub xub ntiag ntawm hydrogen peroxide thiab peroxidase, tus tshiab Trinder'si reagent tuaj yeem siv ua ke nrog 4-aminoantipyrine (4-AA) lossis 3 methyl Thaum lub sij hawm oxidative coupling cov tshuaj tiv thaiv ntawm benzothiazole sulfonehydrazone (MBTH), ruaj khov heev ntshav lossis xiav dyes. raug tsim.Cov molar absorbance ntawm cov dyes ua ke nrog MBTH yog 1.5-2 zaug siab dua li cov dyes ua ke nrog 4-AA;Txawm li cas los xij, 4-AA Cov tshuaj yog qhov ruaj khov dua li cov tshuaj MBTH.Lub substrate yog enzymatically oxidized los ntawm nws oxidase los tsim hydrogen peroxide.Qhov concentration ntawm no hydrogen peroxide sib haum mus rau qhov concentration ntawm substrate.Yog li ntawd, tus nqi ntawm cov substrate tuaj yeem txiav txim siab los ntawm kev tsim cov xim ntawm cov tshuaj tiv thaiv oxidative coupling.Glucose, cawv, acyl-CoA, thiab cov roj cholesterol tuaj yeem siv los txheeb xyuas cov substrates ua ke nrog cov tshiab Trinder's reagents thiab 4-AA.Muaj 10 tus tshiab Trinder's reagents.Ntawm cov tshiab Trinder's reagents, TOOS yog feem ntau siv.Txawm li cas los xij, rau ib lub substrate tshwj xeeb, kev sim ntau hom tshiab Trinder's reagents yog tsim nyog los tsim kom muaj kev pom zoo.
