Cov khoom

Ib txoj hauv kev kho mob tseem ceeb rau kev kho mob ntshav qab zib hom 2 yog kev siv cov tshuaj uas tuaj yeem txo qis postprandial hyperglycemia los ntawm inhibiting carbohydrate digesting enzymes.Txoj kev tshawb fawb tam sim no tau tshawb xyuas qhov cuam tshuam ntawm bioassay-guided extract thiab feem ntawm cov txiv hmab txiv ntoo qhuav pericarp ntawm Phaleria macrocarpa, ib txwm siv tshuaj tiv thaiv kab mob ntshav qab zib, ntawm α-glucosidase thiab α-amylase, hauv kev sib tw kom nkag siab txog lawv cov kev tiv thaiv kab mob ntshav qab zib, xws li Ntxiv rau lawv qhov ua tau kom txo qis ntawm cov piam thaj tom qab nce ntxiv.Methanol extract (ME), tau los ntawm kev ua tiav cov kuab tshuaj extraction, nws cov txiaj ntsig zoo tshaj plaws ntawm cov kua n-butanol feem (NBF) thiab flash kem chromatographic sub-fraction (SFI), tau soj ntsuam. rau hauv vitro α-glucosidase (poov tshuaj) thiab α-amylase (porcine) kev ua haujlwm inhibition.Tsis tas li ntawd, kev kuaj pom tseeb hauv vivo tau ua tiav hauv streptozotocin-induced ntshav qab zib nas (SDRs) siv qhov ncauj qabzib, sucrose thiab starch kam rau ua.Ntawm qhov siab tshaj plaws ua haujlwm (1 00 μg / ml), NBF pom tias muaj kev tiv thaiv siab tshaj plaws tiv thaiv α-glucosidase ( 75%) thiab α-amylase (87%) nyob rau hauv vitro (IC50 = 2.40 ± 0.23 μg / ml thiab 58.50 ± 0.13 μg / ml, raws li) nyob rau hauv ib koob tshuaj tiv thaiv;Cov txiaj ntsig pom muaj li ntawm 20% siab dua acarbose (55%), tus qauv α-glucosidase inhibitor (IC50 = 3.45 ± 0.19 μg / ml).ME thiab SFI kuj tseem inhibit α-glucosidase (IC50 = 7.50 ± 0.15 μg / ml thiab 11.45 ± 0.28 μg / ml) thiab α-amylase (IC50 = 43.90 ± 0.19 μg / ml thiab μg 69.80), tab sis tsawg dua.Hauv kev tshawb fawb vivo nrog cov nas mob ntshav qab zib, NBF thiab SFI tau txo qis cov ntshav qabzib siab siab (PBG) los ntawm 15.08% thiab 6.46%, thiab thaj chaw hauv qab qhov kev ua siab ntev (AUC) los ntawm 14.23% thiab 12.46%, feem, tom qab kev sib tw ntawm qhov ncauj sucrose. (P <0.05);li no validating qhov pom nyob rau hauv vitro ua.Cov kev txo qis ntawm PBG thiab AUC kuj tau pom nyob rau hauv kev kuaj ntshav qabzib thiab cov hmoov txhuv nplej siab, tab sis kom qis dua.Cov kev tshawb pom no qhia tias P. macrocarpa tuaj yeem txo qis hyperglycemia hauv ob qho tib si hauv vitro thiab hauv vivo los ntawm muaj zog inhibiting carbohydrate hydrolysing enzymes, ua rau nws. cov nroj tsuag siv tau los muab cov khoom sib xyaw ntuj rau kev tswj hwm hom 2 mob ntshav qab zib mellitus.