Cov khoom

Tus tshiab Trinder's reagents yog cov dej-soluble aniline derivatives uas tau siv dav hauv kev tshuaj ntsuam xyuas thiab kuaj biochemical.Muaj ntau qhov zoo dua li cov pa chromogenic reagents hauv kev txiav txim siab colorimetric ntawm hydrogen peroxide kev ua.Tus tshiab Trinder's reagents ruaj khov txaus los siv rau hauv ob qho tib si kev daws teeb meem thiab kev sim ntsuas cov kav dej.Nyob rau hauv lub xub ntiag ntawm hydrogen peroxide thiab peroxidase, qhov tshiab Trinder's reagent tau pom tias muaj 4- aminoantipyrine (4-AA) los yog 3- methylbenzothiazole sulfonehydrazone (MBTH) thaum lub sij hawm oxidative coupling cov tshuaj tiv thaiv.Cov ntaub ntawv ruaj khov heev violet lossis xiav dyes.Cov molar absorbance ntawm cov dye ua ke nrog MBTH yog 1.5- 2 zaug siab dua li cov xim ntawm cov xim nrog 4-AA;Txawm li cas los xij, cov tshuaj 4-AA tau ruaj khov dua li cov tshuaj MBTH.Lub substrate yog enzymatically oxidized los ntawm nws oxidase los tsim hydrogen peroxide.Qhov concentration ntawm hydrogen peroxide sib haum mus rau lub substrate concentration.Yog li ntawd, tus nqi ntawm substrate tuaj yeem txiav txim siab los ntawm kev tsim cov xim ntawm cov tshuaj tiv thaiv oxidative coupling.Glucose, cawv, acyl-CoA thiab cov roj cholesterol tuaj yeem siv los kuaj xyuas cov substrates ua ke rau cov tshiab Trinder's reagent thiab 4-AA.Muaj 10 tshiab Trinder's reagents muaj.Ntawm cov tshiab Trinder's reagents, TOOS yog feem ntau siv.Txawm li cas los xij, rau ib qho tshwj xeeb substrate, kev sim cov chav sib txawv ntawm cov tshiab Trinder's reagents yog tsim nyog los tsim kom muaj kev pom zoo.