Tom ntej: 4-Nitrophenyl-alpha-D-galactopyranoside CAS: 7493-95-0 99%
| Catalog Number | XD900018 |
| Khoom npe | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Molecular Formula | C12H15NO8 |
| Molecular Luj | 301.25 Nws |
| Cov ntaub ntawv khaws cia | -15 rau -20 ° C |
| Harmonized tariff Code | 29 400 000 IB |
Khoom Specification
| Qhov tshwm sim | Dawb rau txiv kab ntxwv rau ntsuab hmoov rau siv lead ua |
| Assay | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) yog ib qho khoom siv substrate ntawm 4-nitrophenyl (pNP) glycopyranoside rau kev tshawb nrhiav ntawm α-galactosidase kev ua haujlwm.Tus nqi tso tawm pNP tau nce ntxiv thaum 4-Nitrophenyl α-D-galactopyranoside tau siv los ua ib qho substrate.
Kev siv 4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenylα-D-galactopyranoside yog qhov tseem ceeb tshaj qhov tseem ceeb tshaj plaws los ntawmtheotherpNP-glycopyranosidesatpH4.0whereasnoactivityCov. -nitrophenyl (pNP) glycopyranosidesasartificialsubstrates qhia tau hais tias muaj kev sib koom ua ke hauv kev sib txuas ntawm galactoseresiduesfrom4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, iethepHlevelwhereyieldthresholdis txo qis dua.
Substrate ntawm α-D-galactosidase (α-D-Galactosidase).khi rau E. coli lactose protein carrier.Nruab nrab rau α-D-galactose.
Ib txoj hauv kev sai rau kev ntsuam xyuas cov haujlwm lytic ntawm cov tshuaj tiv thaiv kab mob tiv thaiv cov poov xab thiab fungi tau tsim.Qhov kev ntsuam xyuas yog ua raws li kev tso tawm ntawm intracellular enzyme, maltase (alpha-glucosidase).Cov kev ua maltase tso tawm tau ntsuas colorimetrically los ntawm kev tsim p-nitrophenol los ntawm p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Cov kev ua lytic ntawm cov tshuaj tua kab mob sib txawv tau ntsuas tawm tsam cov poov xab los yog cov kab mob spores ntawm filamentous fungi.Lytic tiv thaiv kab mob tuaj yeem kuaj pom hauv 20 min incubation ntawm 30 degrees C tiv thaiv Saccharomyces cerevisiae, Candida albicans, thiab Cryptococcus neoformans.Lytic tiv thaiv kab mob tshwm sim tom qab 2 teev ntawm incubation ntawm 30 degrees C tiv thaiv germinating spores ntawm Aspergillus niger thiab Botrytis cinerea.Tag nrho cov hlwb ntawm cov poov xab los yog fungi tsis hydrolyze txaus PNPG nyob rau hauv 3 h ntawm 30 degrees C kom tawm tau cov xim hloov tau.Lytic kev ua haujlwm ntawm cov enzymes (xws li Lyticase), tshuaj tua kab mob (xws li, Amphotericin B), thiab cov kab mob uas tsim cov tshuaj tua kab mob tau kuaj pom siv qhov kev xeem.Cov tshuaj tiv thaiv kab mob tau raug hloov mus rau 96-zoo microtiter hom.Ob qhov kev soj ntsuam tau muab kev tshawb nrhiav sai, nkag siab, thiab rov tsim dua tshiab ntawm lytic los tiv thaiv cov poov xab thiab tiv thaiv cov kab mob fungal.
