Cov khoom

Chemifluorescence: Molecular luminol yog cov tshuaj fluorescent molecule uas tuaj yeem hloov mus rau hauv lub xeev zoo siab aminophthalic acid nyob rau hauv muaj hydrogen peroxide molecules, uas emits muaj zog fluorescence.Hydrogen peroxide yog cov khoom lag luam ntawm ntau cov tshuaj biooxidative, yog li nws yooj yim los txuas cov biooxidative cov tshuaj tiv thaiv nrog photodetection los ntawm kev qhia luminol.Piv txwv li, cov piam thaj oxidase / catalase sojntsuam tuaj yeem kuaj xyuas qhov concentration ntawm hydrogen peroxide lossis qabzib hauv cov qauv, thiab lub sijhawm teb tsuas yog 0.5s (txoj kev dynamic).Kev sib xyaw cov tshuaj chemiluminescent nrog cov tshuaj tiv thaiv kab mob, thiab nthuav tawm cov ntsiab lus ntawm cov tshuaj tiv thaiv kab mob los ntawm cov tshuaj tiv thaiv lub teeb, hu ua chemiluminescent immunoassay technology.Nyob rau hauv 1976, Shmeder tau npaj chemiluminescence immunoassay, siv luminol-H202 thiab nws cov derivative ABEI raws li kev kuaj pom cov tshuaj tiv thaiv.Tam sim no, luminol thiab nws cov derivatives yog cov cim siv ntau tshaj plaws hauv cov ntawv thov chemiluminescence immunoassay.Nyob rau hauv cov xwm txheej alkaline, catalyzed los ntawm microperoxidase, ntau cov photons tuaj yeem raug tso tawm.Luminol tuaj yeem siv rau WesternBlot ntawm HRP-labeled antibodies thiab nucleic acid hybridization nrhiav pom ntawm HRP-labeled probes.Nws kuj yog siv nyob rau hauv niaj hnub criminal detective bloodstain detection.Ib tug compound uas yuav siv tau raws li ib tug chemiluminescent marker yuav tsum tau raws li nram no tej yam kev mob: lub quantum yield ntawm luminescence yog siab;nws cov khoom physicochemical zoo sib xws hauv kev kawm;nws cov tshuaj tiv thaiv luminescent yog qhov tshwm sim ntawm cov tshuaj tiv thaiv oxidation ntawm cov khoom luminescent;nyob rau hauv lub concentration ntau siv Internally non-toxic rau cov kab mob nyob.Ntau hom chemiluminescence reagents feem ntau yog siv: acridine ester, dav siv chemiluminescence tracer, nws yog tricyclic organic compound, yooj yim rau oxidize, thiab cov tshuaj tiv thaiv oxidation tsis tas yuav muaj catalyst, thiab tso tawm photons ntawm 430nm;Luminol thiab isoluminol thiab lawv cov derivatives yog cov neeg ua haujlwm zoo tshaj plaws chemiluminescent.Thaum ntxov li 1964, Thaum qhia txog chemiluminescence, luminol, isoluminol thiab lawv cov derivatives tau siv rau chemiluminescence.Immunoassays tau ua tiav cov txiaj ntsig zoo dua.Luminescence nrhiav pom: qhov zoo tshaj plaws fluorescence wavelength yog 400nm (nrhiav cov chemiluminescence hauv 60mmK2S2O8, 100mmK2CO3, pH11.5 kua)

Chemiluminescence: Raws li kev soj ntsuam rhiab heev, chemiluminescence yog dav siv los kuaj cov dawb radicals thiab cov tshuaj tiv thaiv metabolites uas tsim tawm hauv cov enzymes, hlwb thiab cov kab mob lom.Lub teeb tawm los ntawm lawv cov khoom oxidation thiab metabolites tuaj yeem siv nrog ntau yam photometers.kuaj pom.Chemiluminescence yog dav siv nyob rau hauv kev tshuaj ntsuam thiab kev tshawb fawb ntawm cov tshuaj antioxidants, xws li polyphenols, polysaccharides, flavonoids, thiab anthraquinones, vim nws rhiab heev, ceev ceev, kev khiav hauj lwm yooj yim thiab tus nqi qis.Feem ntau siv chemiluminescence systems rau kev txiav txim siab ntawm superoxide anions suav nrog xanthine oxidase-luminol, pyrogallol-luminol thiab dimethyl sulfoxide-luminol.Lub qub yog ib qho enzymatic system, thiab ob lub tom kawg Nws yog ib qho tsis-enzymatic system.Cov txheej txheem chemiluminescence rau kev txiav txim siab ntawm hydroxyl radicals feem ntau suav nrog tooj liab sulfate-pob txha (los yog cov hlwb pob txha)-ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate tsis, tooj liab sulfate-o-phenanthroline-ascorbic acid-hydrogen. peroxide 5 chemiluminescence systems, ferrous sulfate-luminol-hydrogen peroxide thiab ferrous sulfate-luminol, tag nrho cov koom nrog cov classic Fenton cov tshuaj tiv thaiv los tsim hydroxyl radicals, thiab tom qab ntawd tawm tsam cov luminescent tus neeg sawv cev los tsim chemiluminescence, uas tuaj yeem siv los ntsuas qhov kev tshem tawm ntawm cov extracts. kev ua haujlwm ntawm reactive oxygen radicals.Kev sim txoj kev: siv pyrogallol-luminol chemiluminescence system: luminol yog tsim rau hauv 0.05mol / L concentration tov nrog 0.05mol / L NaOH tov, muab cia rau hauv ib qho chaw tsaus, thiab ob chav distilled dej ua ntej siv Dilute rau 1mmol / L tov, siv 1mmol / L HCl los ua 0.01mol / L tov ntawm pyrogallol, muab cia rau hauv lub tub yees ntawm 4 ° C, thiab dilute nws nrog ob distilled dej mus rau 16 zaug 6.25 × 10-4mol / L tov ua ntej siv.0.05mol/L pH10.2Na2CO3-NaHCO3 buffer (muaj 0.1mmol/LEDTA) tau npaj ua ntej siv, thiab tov nrog 1mmol/L luminol ntawm 2:1 (Volume fraction) los tsim luminol thiab carbonate tsis ua ntej kev sim sib tov.Thaum lub sij hawm ntsuas, txhaj 10.0 μL cov qauv ntawm qhov sib txawv (0, 0.08, 0.4, 2 thiab 10 mg / mL) rau hauv luminescent cell (nrog rau cov qauv tsis raws li kev tswj), thiab tom qab ntawd txhaj 6.25 × 10-4mol / L pyrogallol 0.05 Thaum kawg, 0.94 mL ntawm kev sib xyaw ntawm luminol thiab carbonic acid tsis tau ntxiv los pib cov tshuaj tiv thaiv (30 ° C), qhov luminescence siv tau raug suav nrog ntawm 2 s, thiab tag nrho cov kev sib xyaw luminescence siv ntawm 300 s raug ntsuas.Lub keeb kwm yav dhau luminescence siv yog lub luminescence thaum pyrogallol tsis ntxiv.tus nqi.Tsis tas li ntawd, muaj chemiluminescence, fluorescence spectroscopy thiab NBS-dichlorofluorescein chemiluminescence system rau kev txiav txim siab ntawm fluorescence chemiluminescence, thiab lwm yam, lawv cov txiaj ntsig zoo tshaj plaws yog siab rhiab heev.

Chemical Properties: Yellow crystalline hmoov.Yooj yim soluble hauv lye, soluble hauv dilute acid, yuav luag insoluble hauv dej, thiab insoluble hauv cawv.Cov kev daws teeb meem nruab nrab lossis me ntsis acidic pom muaj zog ci xiav fluorescence thaum raug ultraviolet lub teeb.Melting point 329-332 ℃

Siv: Raws li tshuaj ntsuam xyuas reagent thiab ntsuas.Siv Chemiluminescence tsom xam nrhiav reagent (xws li kev txiav txim siab ntawm cov hlau cations lossis ntshav)

Siv: Rau chemiluminescence tsom xam, xws li: hlau cations, ntshav thiab glucocorticoids

Siv: Luminescence test: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; tom qab ntxiv H2O2) chemiluminescence reagent thiab ntsuas, feem ntau siv hauv chemiluminescence tsom xam, xws li hlau cations, ntshav tiv thaiv kab mob