3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Catalog Number | xwm 90075 |
Khoom npe | 3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate |
CAS | 75621-03-3 |
Molecular Formula | C32H58N2O7S |
Molecular Luj | 614.88 ib |
Cov ntaub ntawv khaws cia | Ambient |
Sib raug zoo tariff Code | 29242970 ib |
Khoom Specification
IR | Piv rau siv |
Poob rau ziab | <2% |
TLC | Ib qho chaw |
Nitrogen | 4.46-4.65 Nws |
Qhov tshwm sim | Dawb hmoov |
Solubility (10% hauv H2O) | Ntshiab thiab tsis muaj xim |
Conductivity (0.1M hauv H2O) | <10 us/cm |
Kev ntsuam xyuas HPLC | > 98% |
UV (5% H2O) A290nm | <0.5 |
UV (5% H2O) A400nm | <0.02 |
Cov ntsiab lus hnyav (los ntawm GC) Dimethylformamide | <2.0% |
3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid sab hauv ntsev siv thiab kev sib txuas
Kev Ua Txhaum Cai: CHAPS, ib qho derivative ntawm Cholicacid, yog zwitterionic detergent uas solubilizes membrane proteins.CHAPS yog siv los txhim kho ntau yam protein-DNA complexes thiab tuaj yeem khaws cov kev ua haujlwm biochemical ntawm cov proteins hauv kev daws.
Siv: CHAPS yog ib qho tsis-denaturing zwitterionic detergent rau membrane biochemistry.Nws tuaj yeem siv los solubilize membrane proteins thiab cuam tshuam kev sib cuam tshuam ntawm cov protein-protein.CHAPS muaj qhov hnyav micellar molecular qis (6,150) thiab qhov tseem ceeb micelle concentration (6-10 mM) thiab tuaj yeem raug tshem tawm ntawm cov qauv los ntawm kev lim ntshav.Nws kuj yog tsim rau solubilizing proteins nyob rau hauv isoelectric focusing thiab 2D electrophoresis.CHAPS feem ntau yog siv rau hauv haiv neeg (tsis muaj urea-dawb) isoelectric tsom thiab tau pom tias muab kev daws teeb meem zoo rau qee qhov kev npaj subcellular thiab cog cov proteins.Feem ntau cov concentration ntawm 2-4% (w / v) yog siv rau isoelectric focusing gels.
Siv: Ib qho tsis-denaturing zwitterionic detergent rau membrane biochemistry, uas tuaj yeem siv los solubilize membrane proteins thiab cuam tshuam kev sib cuam tshuam ntawm cov protein-protein;kuj haum rau solubilizing proteins nyob rau hauv isoelectric focusing thiab ob-dimensional electrophoresis;feem ntau siv hauv Non-denaturing (tsis muaj urea) isoelectric tsom;RNA thiab DNA rho tawm, tsis muaj RNase thiab DNase kev ua haujlwm
Siv: Amphoteric surfactant, CHAPS yog cov tshuaj tsis muaj zog zwitterionic detergent, protein lysate rau solubilizing membrane proteins thiab lysing kev sib cuam tshuam ntawm cov protein thiab cov proteins.